Well there are some positive and some negative results from yesterdays time-lapse imagery test.
The imagery above shows a group of cells initially developing (you can see one long thin cell divide at about 8 seconds in) but then, they fall into a decline, eventually floating away, dead. I did this test with some pig bladder smooth muscle cells which I have been successfully maintaining in the lab for several months and as a control I placed a flask from the same batch in a normal incubator and I also placed one in the same hot room environment, but outside of the modified sealed box unit where I have been adding the CO2 enriched air.
The medium in both of the cell flasks which were in the hot room environment is a tell-tale dark pink colour indicating that there has been a significant change in the pH level overnight; the flask which was in the incubator is a healthier orange-pink.
It is clear that the system I have put in place for providing CO2 (attaching the canister of CO2 enriched air and attempting to fill the sealed container) is still not sophisticated enough to keep the cells alive.
There are however, some positive things to take away from the experiment:
Tomorrow I’ll see how this test can be optimised – it is all about incrementation!