Pigs Bladder Football

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This article was written on 17 Jul 2012, and is filled under 2012, Cell Materials, Lab, News, time lapse.

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Time Lapse Imagery Results #1

Well there are some positive and some negative results from yesterdays time-lapse imagery test.

The imagery above shows a group of cells initially developing (you can see one long thin cell divide at about 8 seconds in) but then, they fall into a decline, eventually floating away, dead. I did this test with some pig bladder smooth muscle cells which I have been successfully maintaining in the lab for several months and as a control I placed a flask from the same batch in a normal incubator and I also placed one in the same hot room environment, but outside of the modified sealed box unit where I have been adding the CO2 enriched air.

The medium in both of the cell flasks which were in the hot room environment is a tell-tale dark pink colour indicating that there has been a significant change in the pH level overnight; the flask which was in the incubator is a healthier orange-pink.
It is clear that the system I have put in place for providing CO2 (attaching the canister of CO2 enriched air and attempting to fill the sealed container) is still not sophisticated enough to keep the cells alive.

There are however, some positive things to take away from the experiment:

  1. The time-lapse imagery has worked well.  I had forgotten to fix the white balance level so there is some variation in the tone of the images over the course of the short video, but aside from that the focus and contrast seem ok.  Also – the framerate seems quite good – there is much more subtle movement among the cells than I had anticipated and this is quite interesting to watch – I think even taking a shot every 30 seconds (as opposed to 1 frame per minute, which this is) would even work.
  2. I placed a beaker of water inside the sealed container and this seems to have done the trick regards humidity because, if we look at the control-flask which was just in the hot-room environment we can see significant condensation inside.  This would dehydrate the cells over time.
  3. Most importantly, when I observed each flask after, the incubated flask had progressed slowly (in terms of growth) during the 24 hours and in the flask from the hot room no cells were visibly adherent to the flask – all were floating as debris, however in our test flask their were still one or two viable cells which had survived – so the C)2 is a step in the right direction (obviously of course) but we just need a better way to regulate it now.

Tomorrow I’ll see how this test can be optimised – it is all about incrementation!

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